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Dextran gel
The full name is hydroxypropyl glucan gel. Sephadex lh-20 is the product of Sephadex g-25 (one of the commercial models of dextran gel according to crosslinking degree) after hydroxypropyl treatment. At this time, the glucose part of dextran gel molecule will combine with hydroxypropyl into the following ether bond form: -oh → -och2ch2ch2oh. Compared with Sephadex G, the total number of -oh groups in Sephadex lh-20 molecules did not change, but the proportion of carbon atoms was relatively increased. Therefore, Sephadex lh-20 can be used not only in water, but also in polar organic solvents or mixed solvents composed of them and water.
Dextran gel
GE Dextran gel(LH-20)
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Sephadex LH-20葡聚糖凝胶
Sephadex葡聚糖凝胶是通过表氯醇交联多个葡聚糖得到的。Sephadex G-10、G-25这些葡聚糖凝胶是在水溶液中使用,若要用有机溶剂作为流动相,只有通过羟丙基化将Sephadex G转化为可在有机溶剂中溶涨的葡聚糖凝胶。这时的产品为Sephardex LH-20。
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