Quiksep Autobio automatic chromatography system is highly automated, which can be used for intensive programming method exploration, with powerful software function, and hardware supporting a variety of specifications of chromatography columns, multiple solvents and samples. The system adopts modular, functional and intelligent design, which is convenient for customers to choose the most suitable configuration according to their actual needs.
General characteristics
Ø Stackable pipeline design to achieve the minimum dead volume of pipeline;
Ø Meet user customization, realize flexible configuration and easy upgrade;
Ø All valves, plunger pumps, detectors, etc. are modularized. During maintenance or replacement, only the corresponding modules need to be removed;
Ø Self developed control software, FDA compliant, real-time data log record, traceable, non modifiable; remote control;
Ø Material conforming to USP class VI, stainless steel shell, protection class IP54.
Flow path design
In this system, the flow path minimization is adopted, the devices are very compact, and the dead volume of the whole flow path is very small. The flow path is connected and fixed by standard hand tight joint, which is convenient and fast, while greatly reducing the dead volume of the system.
QuikSep AutoBio High configuration flow diagram
QuikSep AutoBio Flow diagram of medium distribution type
QuikSep AutoBio Flow diagram of low distribution type
software design
1）Reasonable and intuitive software operation interface
The software of the chromatography system conforms to the new GMP specification. It adopts graphical interface and full-automatic operation. It is concise and concise. Any operation and status can be displayed in the main interface status bar in real time. It has high automation level, fits the production practice and reduces the technical requirements for the R & D personnel.
（高配型，供参考）
2）Concise and powerful method editing and automatic control function
It can realize automatic collection according to UV, conductivity value, etc.; it can save temporary editing method automatically; it can run automatic process based on time, volume and column volume; it adopts block method to edit, which makes the method have the function of jump, interrupt and nesting. With strong watch instruction, it can realize many functions, including automatic sample loading, automatic collection and bubble detection Automatic switching and other functions.
3）Manual operation or method operation
The basic operation information will be displayed in the operation dialog window. Manual operation and method operation are optional, and the result saving path can be customized.
4）Convenience of operation
Process control is point and use. It can not only check the log and operation method in real time, but also calculate the curve online.
5）Curve setting
You can customize the curve display color of each parameter, as well as the dotted line and solid line. You can set the y-axis display range of each parameter, or select Auto to set the x-axis display unit (time / volume / CV) and display range.
6）Scouting function
Scouting the exploration function, by editing some parameters in the method into variables, and then running the same method multiple times through scoring setting, each time only automatically modifying one or some parameters in the method, so as to realize the automatic optimization of parameters, such as finding the most suitable sample flow rate, elution gradient, etc.
The scoring function only needs to set variables and scoring times simply, which can not only realize repeated operation of the method, fully automatic process optimization, but also realize automatic preparation of multiple eluents, and realize the maximum intelligence of the instrument.
7）Rights management function
The system adopts five levels of user management authority, which can be assigned by users themselves, and users can be changed during operation (convenient for shift handover).
8）Audit tracking and electronic signature function
Perfect audit tracking and electronic signature functions, in line with GMP regulations.
9）Result analysis and data storage function
The data can be saved in real time, even if the power is cut off, the data will not be lost; the column effect analysis can be carried out for the atlas, and the baseline can be adjusted manually; the system log and method information can be recorded in real time, and the curve comparison can be carried out for the convenience of tracing.
10）Pdf report can be customized
Through the wizard, it is convenient to generate standardized chromatography results report, and to copy and organize data.
Main components
1）Plunger pump
It is made of sanitary material and has good compatibility with biological samples such as protein;
It can not only withstand high pressure, but also has good stability under low temperature and low pressure, and has excellent infusion accuracy;
The pump head has a self-cleaning function to avoid the separation of salt in the pump head during the purification of biological samples, resulting in instrument damage and pollution;
It can provide excellent gradient accuracy and repeatability, and ensure the reproducibility of purification results.
2）detector
UV detector adopts imported device, variable dual wavelength, to ensure good accuracy and stability.
The pressure sensor adopts international famous brand with high precision, which can provide perfect protection for packing and system pump.
PH / conductivity adopts international famous brand, which can be calibrated online.
3）valve
Adopt the famous brand multi-channel solenoid valve, the minimum dead volume design.
Modular design of valve head and drive part, standard hand tight joint fixed pipeline, easy to maintain.
4）Component collector
Component collector is a new generation of microcomputer control instrument for laboratory automation, which can collect the automatic components of chromatography liquid in the tube. The instrument has the characteristics of stable and reliable performance, simple operation, wide application range, small volume and light weight.
The component collector has reasonable structure and flexible action, and adopts the original motion principle: the combination of linear motion and rotary motion; the collector can reach any collection position as quickly as possible. The unique structure ensures the maximum use of space, making the equipment compact and easy to use; especially when it is used with quiksep Autobio chromatography system, it is more flexible and does not interfere with each other.
Click "tools" → "component collector" in the operation interface to open the collector operation interface, as shown in the following figure:
A scientific research institute in Xi'an
A protein medicine enterprise in Shaanxi Province
