Preparation of chromatographic column by quiksep DAC dynamic axial compression
Quiksep DAC dynamic axial compression column is easy to use, users can fill the required packing by themselves, with high efficiency, high column efficiency, good symmetry and repeatability, and long service life. It can be filled with packing as small as 10 μ m, and can achieve almost the same high column efficiency as the analytical column. The unique design of distributor and collector is the most effective guarantee. It is suitable for the extraction of natural plants, the separation and preparation of synthetic drugs, proteins and polypeptides, and it is also an essential means for the modernization of traditional Chinese medicine.
Reasons for selecting quiksep DAC dynamic axial compression to prepare chromatographic column
1. After sales service of gold medal, we promise not only the product quality, but also your equipment use and method development;
2. Good reproducibility, high column efficiency, good symmetry, good for linear amplification;
3. Focus on chromatographic analysis, purification and separation for ten years;
4. Meet GMP and FDA requirements;
Product features
1. Using DAC dynamic axial compression technology, the piston can rise and fall quickly and stably, with good pressure stabilizing effect and long pressure stabilizing time, avoiding the collapse of the inlet end to form a hole and causing the liquid to occur in the hole. 2. Strong local convection and sample diffusion can keep the column effect stable for a long time and avoid the loss of column effect;
3. Adopt static and dense mechanical polishing, electropolishing and cylindricization technology to minimize the pipe wall effect in the process of column installation and use and avoid channeling phenomenon, which conforms to GMP and FDA specifications;
4. The dynamic seal design of the pressurized piston body of chromatographic column adopts redundant double seal, TFL spring real and O-ring of EPDM certified by FDA to ensure zero leakage of piston;
5. The unique design of liquid distribution system makes the column efficiency, column pressure and symmetry perfectly unified, with better reproducibility;
6. Pneumatic pressure control to meet the requirements of special explosion-proof operation environment.
7. Provide customized design services for products with special requirements.
Piston distributor
The piston distributor adopts the patented technology dynamic axial compression column fluid distribution device, and the advanced h-tree distribution model is carved on the piston body. By using polytetrafluoroethylene as the transition plate, the secondary distribution is realized, and the h-tree distributor is refined to more than eight levels, so that the liquid entering each cross-section of the piston is still on the same cross-section when it reaches the surface of the piston sieve plate, avoiding the sample diffusion in the solvent, so as to ensure the column efficiency of the preparation column.
Column bottom collector
The column bottom collector also adopts patented technology. The fast collector is designed on the principle of hydrodynamics. The angle between the main flow lines of liquid is 45 degrees. The angle between the branch flow line and the main flow line is 60 degrees. Under high pressure, the liquid will naturally flow in the direction of low pressure. This design provides the shortest circuit for the flowing liquid, reduces the dead volume, and reduces the tailing phenomenon under the condition of using ultraviolet detector.
The system is designed on the basis of absorbing foreign technology. The system is equipped with DAC preparative high-pressure chromatographic column, high-precision preparative high-pressure infusion pump, UV-Vis detector which can realize on-line real-time detection, independently developed chromatographic workstation control software, etc., and can also be used in combination with on-line filter, static / dynamic mixer, fraction collector, etc., which can meet the needs of separation and purification in different scales;
The system has the characteristics of high resolution, good selectivity, continuous elution of cocoa, high yield, high purity of active ingredients and easy operation. It is suitable for the separation and purification of natural products, bioengineering, genetic engineering, protein purification, pharmaceutical industry and fine chemical industry. It is the core equipment for the separation and purification of active ingredients of Chinese herbal medicine, chemical synthetic drugs and biological active substances.
